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Human Dihydrotestosterone,DHT ELISA Kit

Product Details

Target Name Dihydrotestosterone,DHT
Alternative Names N/A
Abbreviation DHT
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 10 pg/mL-2000 pg/mL
Sensitivity 5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-97  
1:2 Average % 89  
Range % 85-99  
1:4 Average % 93  
Range % 86-105  
1:8 Average % 94  
Range % 87-103  
Recovery
The recovery of human DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-99  
EDTA plasma (n=4) 97 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.179 0.185 0.182    
500 0.313 0.319 0.316    
160 0.523 0.529 0.526    
40 0.818 0.802 0.810    
10 1.317 1.330 1.324    
0 1.952 1.943 1.948    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days