Human DNA-binding protein inhibitor ID-1(ID1) ELISA kit
Product Details
| Target Name |
inhibitor of DNA binding 1, dominant negative helix-loop-helix protein |
| Alternative Names |
bHLHb24 ELISA Kit; Class B basic helix-loop-helix protein 24 ELISA Kit; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) ELISA Kit; DNA binding protein inhibitor ID 1 ELISA Kit; DNA binding protein inhibitor ID1 ELISA Kit; DNA-binding protein inhibitor ID-1 ELISA Kit; Dominant negative helix loop helix protein ELISA Kit; ID 1 ELISA Kit; ID ELISA Kit; ID1 ELISA Kit; ID1_HUMAN ELISA Kit; Inhibitor of Differentiation 1 ELISA Kit; Inhibitor of DNA binding 1 ELISA Kit; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein ELISA Kit |
| Abbreviation |
ID1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ID1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:8 |
Average % |
85 |
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| Range % |
82-93 |
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| Recovery |
| The recovery of human ID1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-95 |
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| EDTA plasma (n=4) |
101 |
96-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.654 |
2.647 |
2.651 |
2.501 |
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| 1000 |
2.222 |
2.327 |
2.275 |
2.125 |
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| 500 |
1.567 |
1.544 |
1.556 |
1.406 |
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| 250 |
0.785 |
0.745 |
0.765 |
0.615 |
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| 125 |
0.437 |
0.433 |
0.435 |
0.285 |
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| 62.5 |
0.358 |
0.347 |
0.353 |
0.203 |
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| 31.25 |
0.246 |
0.254 |
0.250 |
0.100 |
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| 0 |
0.151 |
0.148 |
0.150 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
