Human DNA damage-inducible transcript 4 protein(DDIT4) ELISA kit
Product Details
| Target Name |
DNA-damage-inducible transcript 4 |
| Alternative Names |
DDIT4 ELISA Kit; DDIT4_HUMAN ELISA Kit; Dig2 ELISA Kit; DNA damage inducible transcript 4 ELISA Kit; DNA damage inducible transcript 4 protein ELISA Kit; DNA damage-inducible transcript 4 protein ELISA Kit; FLJ20500 ELISA Kit; HIF 1 responsive protein RTP801 ELISA Kit; HIF 1 responsive RTP801 ELISA Kit; HIF-1 responsive protein RTP801 ELISA Kit; Protein regulated in development and DNA damage response 1 ELISA Kit; REDD-1 ELISA Kit; REDD1 ELISA Kit; RTP801 ELISA Kit |
| Abbreviation |
DDIT4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, tissue homogenates, cell lysates, urine, cerebrospinal fluid (CSF) |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human DDIT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
86-92 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-99 |
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| 1:8 |
Average % |
98 |
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| Range % |
92-103 |
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| Recovery |
| The recovery of human DDIT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.948 |
2.754 |
2.851 |
2.691 |
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| 5 |
2.539 |
2.412 |
2.476 |
2.316 |
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| 2.5 |
1.781 |
1.662 |
1.722 |
1.562 |
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| 1.25 |
1.248 |
1.198 |
1.223 |
1.063 |
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| 0.625 |
0.738 |
0.706 |
0.722 |
0.562 |
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| 0.312 |
0.514 |
0.504 |
0.509 |
0.349 |
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| 0.156 |
0.305 |
0.298 |
0.302 |
0.142 |
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| 0 |
0.162 |
0.158 |
0.160 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
