Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1) ELISA kit
Product Details
| Target Name |
dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| Alternative Names |
156DAG ELISA Kit; A3a ELISA Kit; AGRNR ELISA Kit; Alpha dystroglycan ELISA Kit; Alpha-DG ELISA Kit; Beta-DG ELISA Kit; Beta-dystroglycan ELISA Kit; DAG ELISA Kit; Dag1 ELISA Kit; DAG1_HUMAN ELISA Kit; Dystroglycan 1 (dystrophin associated glycoprotein 1) ELISA Kit; Dystroglycan ELISA Kit; Dystrophin associated glycoprotein 1 ELISA Kit; Dystrophin-associated glycoprotein 1 ELISA Kit; OTTHUMP00000210857 ELISA Kit; OTTHUMP00000210858 ELISA Kit |
| Abbreviation |
DAG1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
125 pg/mL-8000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human DAG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
93-103 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
87-99 |
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| Recovery |
| The recovery of human DAG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
88-97 |
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| EDTA plasma (n=4) |
88 |
81-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 8000 |
2.301 |
2.159 |
2.230 |
2.098 |
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| 4000 |
1.934 |
1.868 |
1.901 |
1.769 |
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| 2000 |
1.562 |
1.534 |
1.548 |
1.416 |
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| 1000 |
1.234 |
1.202 |
1.218 |
1.086 |
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| 500 |
0.825 |
0.845 |
0.835 |
0.703 |
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| 250 |
0.543 |
0.551 |
0.547 |
0.415 |
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| 125 |
0.322 |
0.310 |
0.316 |
0.184 |
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| 0 |
0.133 |
0.131 |
0.132 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
