Human EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit
Product Details
| Target Name |
EGF-containing fibulin-like extracellular matrix protein 1 |
| Alternative Names |
DHRD ELISA Kit; Doyne honeycomb retinal degeneration ELISA Kit; DRAD ELISA Kit; EFEMP 1 ELISA Kit; EFEMP1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 precursor ELISA Kit; EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Epidermal growth factor containing fibulin like extracellular matrix protein 1 ELISA Kit; Extracellular protein S1 5 ELISA Kit; Extracellular protein S1-5 ELISA Kit; FBLN 3 ELISA Kit; FBLN3 ELISA Kit; FBLN3_HUMAN ELISA Kit; FBNL ELISA Kit; FIBL 3 ELISA Kit; FIBL-3 ELISA Kit; FIBL3 ELISA Kit; Fibrillin like ELISA Kit; Fibrillin like protein ELISA Kit; Fibrillin-like protein ELISA Kit; FIBULIN 3 ELISA Kit; Fibulin-3 ELISA Kit; Fibulin3 ELISA Kit; FLJ35535 ELISA Kit; Heat shock 70 KD protein 1 ELISA Kit; MGC111353 ELISA Kit; MLVT ELISA Kit; MTLV ELISA Kit; S1 5 ELISA Kit; T16 protein ELISA Kit |
| Abbreviation |
EFEMP1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
102 |
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| Range % |
98-109 |
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| 1:2 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-97 |
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| Recovery |
| The recovery of human EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
83-94 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.261 |
2.127 |
2.194 |
2.074 |
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| 50 |
1.539 |
1.500 |
1.520 |
1.400 |
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| 25 |
0.854 |
0.902 |
0.878 |
0.758 |
|
| 12.5 |
0.517 |
0.545 |
0.531 |
0.411 |
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| 6.25 |
0.315 |
0.304 |
0.310 |
0.190 |
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| 3.12 |
0.226 |
0.233 |
0.230 |
0.110 |
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| 1.56 |
0.170 |
0.182 |
0.176 |
0.056 |
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| 0 |
0.121 |
0.118 |
0.120 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
