| Description |
The Human Elastin (ELN) ELISA Kit is engineered for accurate measurement of ELN levels from samples, including serum, plasma, cell culture supernates, tissue homogenates, cell lysates, and urine. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the ELN content in the sample. ELN is an essential extracellular matrix (ECM) protein of higher vertebrates and the core protein of elastic fibers. As a structural protein, ELN confers elasticity and resilience to various vertebrate tissues and organs such as lungs, skin, and elastic ligaments. In addition to influencing the architecture and biomechanical properties of the extracellular matrix, ELN also plays a vital role in various physiological processes. Some elastin-derived peptides (EDPs) promote angiogenesis and involved in the modulation of various cell activities, including cell adhesion, chemotaxis, migration, proliferation, protease activation, and apoptosis.
The kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See more details through the product instructions.
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| Target Name |
elastin |
| Alternative Names |
Elastin ELISA Kit; ELN ELISA Kit; ELN_HUMAN ELISA Kit; SVAS ELISA Kit; Tropoelastin ELISA Kit; WBS ELISA Kit; WS ELISA Kit |
| Abbreviation |
ELN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
84 |
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| Range % |
80-100 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:400 |
Average % |
97 |
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| Range % |
92-112 |
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| 1:800 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human ELN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-100 |
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| EDTA plasma (n=4) |
97 |
90-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.702 |
2.934 |
2.818 |
2.717 |
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| 100 |
2.196 |
2.184 |
2.190 |
2.089 |
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| 50 |
1.497 |
1.462 |
1.480 |
1.379 |
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| 25 |
0.885 |
0.876 |
0.881 |
0.780 |
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| 12.5 |
0.477 |
0.486 |
0.482 |
0.381 |
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| 6.25 |
0.244 |
0.256 |
0.250 |
0.149 |
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| 3.12 |
0.152 |
0.161 |
0.157 |
0.056 |
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| 0 |
0.101 |
0.100 |
0.101 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ELN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ELN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
