| Description |
The Human Endothelial nitric oxide synthase (eNOS) ELISA Kit is a sandwich immunoassay specifically designed and validated for the quantitative measurement of eNOS in serum, plasma, cell culture supernates, or tissue homogenates. This assay employs the Sandwich-ELISA technique, in which eNOS in the samples or standards is sandwiched between pre-coated an anti-human CgA and Biotin-labeled eNOS antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of eNOS in the sample. The kit has high sensitivity, excellent specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions. eNOS, also known as nitric oxide synthase 3 (NOS3), is a major physiological source of NO in the vascular endothelium. It exerts a protective role in the cardiovascular system. Up... Read more |
| Target Name |
nitric oxide synthase 3 (endothelial cell) |
| Alternative Names |
cNOS ELISA Kit; Constitutive NOS ELISA Kit; EC NOS ELISA Kit; EC-NOS ELISA Kit; ecNOS ELISA Kit; Endothelial nitric oxidase synthase ELISA Kit; Endothelial nitric oxide synthase ELISA Kit; Endothelial nitric oxide synthase 3 ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; Nitric oxide synthase 3 (endothelial cell) ELISA Kit; Nitric oxide synthase 3 ELISA Kit; Nitric oxide synthase 3 endothelial cell ELISA Kit; Nitric oxide synthase endothelial ELISA Kit; Nitric oxide synthase; endothelial ELISA Kit; NOS 3 ELISA Kit; NOS III ELISA Kit; NOS type III ELISA Kit; NOS3 ELISA Kit; NOS3_HUMAN ELISA Kit; NOSIII ELISA Kit |
| Abbreviation |
NOS3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
1.1 IU/mL-70 IU/mL |
| Sensitivity |
0.27 IU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
|
| Range % |
81-102 |
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| 1:2 |
Average % |
96 |
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| Range % |
84-115 |
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| 1:4 |
Average % |
108 |
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| Range % |
90-110 |
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| 1:8 |
Average % |
95 |
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| Range % |
80-105 |
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| Recovery |
| The recovery of human eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
80-105 |
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| EDTA plasma (n=4) |
94 |
82-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| IU/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 70 |
2.835 |
2.697 |
2.766 |
2.612 |
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| 35 |
1.930 |
1.955 |
1.943 |
1.789 |
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| 17.5 |
1.146 |
1.159 |
1.153 |
0.999 |
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| 8.75 |
0.673 |
0.701 |
0.687 |
0.533 |
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| 4.4 |
0.457 |
0.446 |
0.452 |
0.298 |
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| 2.2 |
0.321 |
0.326 |
0.324 |
0.170 |
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| 1.1 |
0.243 |
0.246 |
0.245 |
0.091 |
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| 0 |
0.156 |
0.152 |
0.154 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human eNOS antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled eNOS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
