Human Eotaxin 2/CCL24 ELISA Kit
Product Details
| Target Name |
chemokine (C-C motif) ligand 24 |
| Alternative Names |
C C motif chemokine 24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; CCL24 ELISA Kit; CCL24_HUMAN ELISA Kit; Chemokine CC Motif Ligand 24 ELISA Kit; CK beta 6 ELISA Kit; CK-beta-6 ELISA Kit; Ckb6 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; MPIF 2 ELISA Kit; MPIF-2 ELISA Kit; MPIF2 ELISA Kit; Myeloid progenitor inhibitory factor 2 ELISA Kit; SCYA24 ELISA Kit; Small inducible cytokine A24 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys) member 24 ELISA Kit; Small-inducible cytokine A24 ELISA Kit |
| Abbreviation |
CCL24 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78.125 pg/mL-5000 pg/mL |
| Sensitivity |
67.660 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-94 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-108 |
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| Recovery |
| The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-103 |
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| EDTA plasma (n=4) |
86 |
83-89 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.152 |
2.204 |
2.178 |
2.065 |
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| 2500 |
1.796 |
1.735 |
1.766 |
1.653 |
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| 1250 |
1.182 |
1.205 |
1.194 |
1.081 |
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| 625 |
0.765 |
0.798 |
0.782 |
0.669 |
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| 312.5 |
0.453 |
0.468 |
0.461 |
0.348 |
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| 156.25 |
0.328 |
0.331 |
0.330 |
0.217 |
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| 78.125 |
0.251 |
0.240 |
0.246 |
0.133 |
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| 0 |
0.115 |
0.110 |
0.113 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
