Human Ephrin type-B receptor 2(EPHB2) ELISA kit
Product Details
| Target Name |
EPH receptor B2 |
| Alternative Names |
cek5 ELISA Kit; Developmentally regulated EPH related tyrosine kinase ELISA Kit; DRT ELISA Kit; EK5 ELISA Kit; ELK related protein tyrosine kinase ELISA Kit; Eph receptor B2 ELISA Kit; EPH tyrosine kinase 3 ELISA Kit; EPH-like kinase 5 ELISA Kit; EPHB2 ELISA Kit; EPHB2_HUMAN ELISA Kit; Ephrin type B receptor 2 ELISA Kit; Ephrin type-B receptor 2 ELISA Kit; EPHT 3 ELISA Kit; ERK ELISA Kit; ETECK ELISA Kit; hEK5 ELISA Kit; Nuk ELISA Kit; Prkm 5 ELISA Kit; Receptor protein tyrosine kinase HEK 5 ELISA Kit; Renal carcinoma antigen NY-REN-47 ELISA Kit; Sek 3 ELISA Kit; Tyro 5 ELISA Kit; Tyrosine protein kinase receptor CEK 5 ELISA Kit; Tyrosine protein kinase receptor EPH 3 ELISA Kit; Tyrosine protein kinase receptor QEK 5 ELISA Kit; Tyrosine-protein kinase receptor EPH-3 ELISA Kit; Tyrosine-protein kinase TYRO5 ELISA Kit |
| Abbreviation |
EPHB2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPHB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:8 |
Average % |
101 |
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| Range % |
96-106 |
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| Recovery |
| The recovery of human EPHB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
88-95 |
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| EDTA plasma (n=4) |
96 |
92-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.607 |
2.688 |
2.648 |
2.462 |
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| 20 |
1.935 |
1.985 |
1.960 |
1.774 |
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| 10 |
1.125 |
1.212 |
1.169 |
0.983 |
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| 5 |
0.683 |
0.698 |
0.691 |
0.505 |
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| 2.5 |
0.442 |
0.447 |
0.445 |
0.259 |
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| 1.25 |
0.309 |
0.323 |
0.316 |
0.130 |
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| 0.625 |
0.230 |
0.246 |
0.238 |
0.052 |
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| 0 |
0.182 |
0.190 |
0.186 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
