Human Epidermal growth factor-like protein 6(EGFL6) ELISA kit
Product Details
| Target Name |
EGF-like-domain, multiple 6 |
| Alternative Names |
EGF like domain multiple 6 ELISA Kit; EGF repeat containing protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; EGF-like-domain multiple 6 ELISA Kit; egfl6 ELISA Kit; EGFL6_HUMAN ELISA Kit; epidermal growth factor like protein 6 ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; MAEG ELISA Kit; MAM and EGF domain containing ELISA Kit; MAM and EGF domains containing gene protein ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; W80 ELISA Kit |
| Abbreviation |
EGFL6 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
104 |
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| Range % |
97-108 |
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| 1:2 |
Average % |
101 |
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| Range % |
93-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
89-101 |
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| 1:8 |
Average % |
104 |
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| Range % |
94-109 |
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| Recovery |
| The recovery of human EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
83-94 |
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| EDTA plasma (n=4) |
102 |
95-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
1.972 |
1.918 |
1.945 |
1.800 |
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| 2500 |
1.400 |
1.439 |
1.420 |
1.275 |
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| 1250 |
1.072 |
1.025 |
1.049 |
0.904 |
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| 625 |
0.762 |
0.734 |
0.748 |
0.603 |
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| 312 |
0.521 |
0.529 |
0.525 |
0.380 |
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| 156 |
0.348 |
0.357 |
0.353 |
0.208 |
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| 78 |
0.271 |
0.283 |
0.277 |
0.132 |
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| 0 |
0.148 |
0.142 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
