Human epidermal growth factor receptor,EGFR ELISA Kit
Product Details
| Target Name |
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Alternative Names |
Avian erythroblastic leukemia viral (v erb b) oncogene homolog ELISA Kit; Cell growth inhibiting protein 40 ELISA Kit; Cell proliferation inducing protein 61 ELISA Kit; EGF R ELISA Kit; EGFR ELISA Kit; EGFR_HUMAN ELISA Kit; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) ELISA Kit; Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) ELISA Kit; Epidermal growth factor receptor ELISA Kit; erb-b2 receptor tyrosine kinase 1 ELISA Kit; ERBB ELISA Kit; ERBB1 ELISA Kit; Errp ELISA Kit; HER1 ELISA Kit; mENA ELISA Kit; NISBD2 ELISA Kit; Oncogen ERBB ELISA Kit; PIG61 ELISA Kit; Proto-oncogene c-ErbB-1 ELISA Kit; Receptor tyrosine protein kinase ErbB 1 ELISA Kit; Receptor tyrosine-protein kinase ErbB-1 ELISA Kit; SA7 ELISA Kit; Species antigen 7 ELISA Kit; Urogastrone ELISA Kit; v-erb-b Avian erythroblastic leukemia viral oncogen homolog ELISA Kit; wa2 ELISA Kit; Wa5 ELISA Kit |
| Abbreviation |
EGFR |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.019 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human EGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
101 |
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| Range % |
95-104 |
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| 1:10 |
Average % |
99 |
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| Range % |
91-103 |
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| 1:20 |
Average % |
94 |
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| Range % |
87-98 |
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| 1:40 |
Average % |
108 |
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| Range % |
97-112 |
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| Recovery |
| The recovery of human EGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
103 |
96-107 |
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| EDTA plasma (n=4) |
93 |
84-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.522 |
2.463 |
2.493 |
2.375 |
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| 10 |
2.018 |
1.977 |
1.998 |
1.880 |
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| 5 |
1.408 |
1.463 |
1.436 |
1.318 |
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| 2.5 |
1.051 |
1.025 |
1.038 |
0.920 |
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| 1.25 |
0.666 |
0.647 |
0.657 |
0.539 |
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| 0.625 |
0.427 |
0.403 |
0.415 |
0.297 |
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| 0.312 |
0.264 |
0.261 |
0.263 |
0.145 |
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| 0 |
0.119 |
0.117 |
0.118 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
