Product Details

Description

The Human Epidermal growth factor (EGF) ELISA Kit is a sandwich immunoassay specifically designed and validated for the quantitative measurement of EGF in serum, plasma, cell culture supernates, or tissue homogenates. Human EGF is a single-chain polypeptide consisting of 53 amino acids. Physiologically in humans, EGF has different innate concentrations in various organs. EGF is implicated in the morphogenesis of teeth, brain, reproductive tracts, skin, gastrointestinal tracts, in cardiovascular differentiation and function, epithelial regeneration, and corneal epithelia. EGF/EGFR signaling pathway regulates proliferation, migration, differentiation, and apoptosis in mammalian cells. Aberrations of the EGF/EGFR signaling pathway will lead to human cancer. This assay employs the Sandwich-ELISA technique, in which eNOS in the samples or standards is sandwiched between pre-coated an anti-human CgA and Biotin-labeled eNOS antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and ...Read more

Target Name

Epidermal growth factor,EGF

Alternative Names

Beta urogastrone ELISA Kit; beta-urogastrone ELISA Kit; EGF ELISA Kit; EGF_HUMAN ELISA Kit; Epidermal growth factor ELISA Kit; HOMG4 ELISA Kit; OTTHUMP00000219721 ELISA Kit; OTTHUMP00000219722 ELISA Kit; Pro epidermal growth factor ELISA Kit; URG ELISA Kit; Urogastrone ELISA Kit

Abbreviation

EGF

Uniprot No.

P01133

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates_x000D_ 

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

0.39 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Materials provided

• A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human EGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
• Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
• One vial Biotin-labeled EGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
• One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
• One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
• One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

Materials not provided

• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate