Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit
Product Details
| Target Name |
chemokine (C-X-C motif) ligand 5 |
| Alternative Names |
AMCFII ELISA Kit; C-X-C motif chemokine 5 ELISA Kit; C-X-C motif chemokine ligand 5 ELISA Kit; Chemokine (C X C motif) ligand 5 ELISA Kit; chemokine (C-X-C motif) ligand 5 ELISA Kit; Cxcl5 ELISA Kit; CXCL5_HUMAN ELISA Kit; ENA 78 ELISA Kit; ENA-78 (8-78) ELISA Kit; ENA-78(1-78) ELISA Kit; ENA-78(9-78) ELISA Kit; ENA78 ELISA Kit; Epithelial derived neutrophil activating protein 78 ELISA Kit; Epithelial-derived neutrophil-activating protein 78 ELISA Kit; Lipopolysaccharide-induced CXC chemokine ELISA Kit; Neutrophil activating peptide ENA 78 ELISA Kit; Neutrophil activating protein 78 ELISA Kit; Neutrophil-activating peptide ENA-78 ELISA Kit; neutrophil-activating protein 78 ELISA Kit; SCYB5 ELISA Kit; Small inducible cytokine B5 ELISA Kit; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78) ELISA Kit; small inducible cytokine subfamily B; member 5 ELISA Kit; Small-inducible cytokine B5 ELISA Kit |
| Abbreviation |
CXCL5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.567 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
94-103 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
86 |
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| Range % |
83-89 |
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| 1:8 |
Average % |
97 |
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| Range % |
94-100 |
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| Recovery |
| The recovery of human ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-89 |
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| EDTA plasma (n=4) |
98 |
94-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.282 |
2.204 |
2.243 |
2.131 |
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| 20 |
1.362 |
1.368 |
1.365 |
1.253 |
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| 10 |
0.781 |
0.757 |
0.769 |
0.657 |
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| 5 |
0.435 |
0.445 |
0.440 |
0.328 |
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| 2.5 |
0.304 |
0.316 |
0.310 |
0.198 |
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| 1.25 |
0.214 |
0.205 |
0.210 |
0.098 |
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| 0.625 |
0.188 |
0.184 |
0.186 |
0.074 |
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| 0 |
0.114 |
0.110 |
0.112 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
