Human Epoxide hydrolase 2(EPHX2) ELISA kit
Product Details
| Target Name |
epoxide hydrolase 2, cytoplasmic |
| Alternative Names |
Bifunctional epoxide hydrolase 2 ELISA Kit; CEH ELISA Kit; Cytosolic epoxide hydrolase ELISA Kit; EPHX2 ELISA Kit; Epoxide hydratase ELISA Kit; Epoxide hydrolase 2 ELISA Kit; Epoxide hydrolase 2 cytoplasmic ELISA Kit; epoxide hydrolase 2, cytosolic ELISA Kit; Epoxide hydrolase soluble ELISA Kit; HYES_HUMAN ELISA Kit; SEH ELISA Kit; Soluble epoxide hydrolase ELISA Kit |
| Abbreviation |
EPHX2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
23.44 pg/mL-1500 pg/mL |
| Sensitivity |
5.86 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPHX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-95 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-97 |
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| Recovery |
| The recovery of human EPHX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
103 |
99-108 |
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| EDTA plasma (n=4) |
93 |
89-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
2.209 |
2.326 |
2.268 |
2.113 |
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| 750 |
1.720 |
1.664 |
1.692 |
1.537 |
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| 375 |
1.194 |
1.213 |
1.204 |
1.049 |
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| 187.5 |
0.871 |
0.857 |
0.864 |
0.709 |
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| 93.75 |
0.546 |
0.532 |
0.539 |
0.384 |
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| 46.88 |
0.383 |
0.401 |
0.392 |
0.237 |
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| 23.44 |
0.257 |
0.249 |
0.253 |
0.098 |
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| 0 |
0.152 |
0.158 |
0.155 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
