Human Fatty acid synthase(FASN) ELISA kit
Product Details
| Target Name |
fatty acid synthase |
| Alternative Names |
[Acyl-carrier-protein] S acetyltransferase ELISA Kit; [Acyl-carrier-protein] S malonyltransferase ELISA Kit; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase ELISA Kit; 3-oxoacyl-[acyl-carrier-protein] reductase ELISA Kit; 3-oxoacyl-[acyl-carrier-protein] synthase ELISA Kit; Enoyl-[acyl-carrier-protein] reductase ELISA Kit; FAS ELISA Kit; FAS_HUMAN ELISA Kit; FASN ELISA Kit; Fatty acid synthase ELISA Kit; MGC14367 ELISA Kit; MGC15706 ELISA Kit; OA 519 ELISA Kit; Oleoyl-[acyl-carrier-protein] hydrolase ELISA Kit; SDR27X1 ELISA Kit; Short chain dehydrogenase/reductase family 27X member 1 ELISA Kit |
| Abbreviation |
FASN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human FASN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
96 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
102 |
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| Range % |
95-108 |
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| 1:8 |
Average % |
88 |
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| Range % |
86-91 |
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| Recovery |
| The recovery of human FASN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
86-95 |
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| EDTA plasma (n=4) |
98 |
94-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.378 |
2.555 |
2.467 |
2.319 |
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| 50 |
1.922 |
1.774 |
1.848 |
1.700 |
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| 25 |
1.401 |
1.499 |
1.450 |
1.302 |
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| 12.5 |
0.989 |
1.059 |
1.024 |
0.876 |
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| 6.25 |
0.616 |
0.627 |
0.622 |
0.474 |
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| 3.12 |
0.355 |
0.344 |
0.350 |
0.202 |
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| 1.56 |
0.274 |
0.288 |
0.281 |
0.133 |
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| 0 |
0.144 |
0.152 |
0.148 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
