Human Fibronectin,FN ELISA Kit
| Target Name |
fibronectin 1 |
| Alternative Names |
CIG ELISA Kit; Cold insoluble globulin ELISA Kit; Cold-insoluble globulin ELISA Kit; DKFZp686F10164 ELISA Kit; DKFZp686H0342 ELISA Kit; DKFZp686I1370 ELISA Kit; DKFZp686O13149 ELISA Kit; ED B ELISA Kit; Fibronectin 1 ELISA Kit; FINC ELISA Kit; FINC_HUMAN ELISA Kit; FN ELISA Kit; FN1 ELISA Kit; FNZ ELISA Kit; GFND ELISA Kit; GFND2 ELISA Kit; LETS ELISA Kit; Migration stimulating factor ELISA Kit; MSF ELISA Kit; Ugl-Y3 ELISA Kit |
| Abbreviation |
FN1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates, cell lysates |
| Detection Range |
12.5 ng/mL-800 ng/mL |
| Sensitivity |
3.12 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:200 |
Average % |
95 |
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| Range % |
88-100 |
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| 1:400 |
Average % |
96 |
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| Range % |
89-103 |
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| 1:800 |
Average % |
93 |
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| Range % |
89-100 |
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| Recovery |
| The recovery of human FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.119 |
2.126 |
2.123 |
2.010 |
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| 400 |
1.797 |
1.704 |
1.751 |
1.638 |
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| 200 |
1.274 |
1.285 |
1.280 |
1.167 |
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| 100 |
0.758 |
0.761 |
0.760 |
0.647 |
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| 50 |
0.482 |
0.495 |
0.489 |
0.376 |
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| 25 |
0.286 |
0.296 |
0.291 |
0.178 |
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| 12.5 |
0.196 |
0.203 |
0.200 |
0.087 |
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| 0 |
0.112 |
0.113 |
0.113 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
