Human Ficolin-2(FCN2) ELISA kit
Product Details
| Target Name |
ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) |
| Alternative Names |
37 kDa elastin-binding protein ELISA Kit; Collagen/fibrinogen domain containing protein 2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; EBP 37 ELISA Kit; EBP-37 ELISA Kit; EBP37 ELISA Kit; FCN 2 ELISA Kit; Fcn2 ELISA Kit; FCN2_HUMAN ELISA Kit; FCNL ELISA Kit; Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) ELISA Kit; Ficolin B ELISA Kit; Ficolin beta ELISA Kit; Ficolin-2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; Ficolin2 ELISA Kit; Hucolin ELISA Kit; L ficolin ELISA Kit; L-ficolin ELISA Kit; OTTHUMP00000022518 ELISA Kit; P35 ELISA Kit; RP11 263F14.2 ELISA Kit; Serum lectin p35 ELISA Kit |
| Abbreviation |
FCN2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.25 ng/mL-16 ng/mL |
| Sensitivity |
0.06 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
86 |
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| Range % |
80-94 |
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| 1:2000 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4000 |
Average % |
108 |
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| Range % |
98-115 |
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| 1:8000 |
Average % |
95 |
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| Range % |
89-98 |
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| Recovery |
| The recovery of human FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
101 |
98-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 16 |
2.007 |
1.900 |
1.954 |
1.844 |
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| 8 |
1.526 |
1.443 |
1.485 |
1.375 |
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| 4 |
1.097 |
1.009 |
1.053 |
0.943 |
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| 2 |
0.645 |
0.621 |
0.633 |
0.523 |
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| 1 |
0.429 |
0.420 |
0.425 |
0.315 |
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| 0.5 |
0.264 |
0.254 |
0.259 |
0.149 |
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| 0.25 |
0.190 |
0.186 |
0.188 |
0.078 |
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| 0 |
0.110 |
0.109 |
0.110 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
