Human Folliculin(FLCN) ELISA kit
Product Details
| Target Name |
folliculin |
| Alternative Names |
BHD ELISA Kit; BHD skin lesion fibrofolliculoma protein ELISA Kit; Birt Hogg Dube syndrome protein ELISA Kit; Birt-Hogg-Dube syndrome protein ELISA Kit; DKFZp547A118 ELISA Kit; FLCL ELISA Kit; Flcn ELISA Kit; FLCN_HUMAN ELISA Kit; FLJ45004 ELISA Kit; FLJ99377 ELISA Kit; Folliculin ELISA Kit; MGC17998 ELISA Kit; MGC23445 ELISA Kit |
| Abbreviation |
FLCN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human FLCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
92 |
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| Range % |
85-98 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-105 |
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| Recovery |
| The recovery of human FLCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
102 |
96-106 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.434 |
2.533 |
2.484 |
2.338 |
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| 500 |
1.695 |
1.598 |
1.647 |
1.501 |
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| 250 |
1.079 |
1.097 |
1.088 |
0.942 |
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| 125 |
0.707 |
0.686 |
0.697 |
0.551 |
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| 62.5 |
0.436 |
0.455 |
0.446 |
0.300 |
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| 31.2 |
0.321 |
0.317 |
0.319 |
0.173 |
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| 15.6 |
0.255 |
0.248 |
0.252 |
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| 0 |
0.147 |
0.145 |
0.146 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
