Human Galectin-1(LGALS1) ELISA kit
Product Details
| Target Name |
lectin, galactoside-binding, soluble, 1 |
| Alternative Names |
14 kDa laminin-binding protein ELISA Kit; 14 kDa lectin ELISA Kit; Beta galactoside binding lectin ELISA Kit; Beta galactoside binding lectin L 14 I ELISA Kit; beta galactoside binding protein ELISA Kit; Beta-galactoside-binding lectin L-14-I ELISA Kit; DKFZp686E23103 ELISA Kit; Gal 1 ELISA Kit; Gal-1 ELISA Kit; GAL1 ELISA Kit; Galaptin ELISA Kit; Galbp ELISA Kit; Galectin ELISA Kit; Galectin-1 ELISA Kit; Galectin1 ELISA Kit; GBP ELISA Kit; HBL ELISA Kit; HLBP14 ELISA Kit; HPL ELISA Kit; L 14.5 ELISA Kit; L-14.5 ELISA Kit; L14 ELISA Kit; Lactose binding lectin 1 ELISA Kit; Lactose-binding lectin 1 ELISA Kit; Lect14 ELISA Kit; Lectin galactoside binding soluble 1 ELISA Kit; Lectin galactoside-binding soluble 1 ELISA Kit; LEG1_HUMAN ELISA Kit; LGALS 1 ELISA Kit; LGALS1 ELISA Kit; Lgals1 lectin galactose binding soluble 1 ELISA Kit; MAPK activating protein MP12 ELISA Kit; Putative MAPK activating protein MP12 ELISA Kit; Putative MAPK-activating protein PM12 ELISA Kit; S Lac lectin 1 ELISA Kit; S-Lac lectin 1 ELISA Kit |
| Abbreviation |
LGALS1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-94 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
97 |
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| Range % |
90-105 |
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| Recovery |
| The recovery of human LGALS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
83-98 |
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| EDTA plasma (n=4) |
94 |
88-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.312 |
2.143 |
2.228 |
2.074 |
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| 100 |
1.953 |
1.823 |
1.888 |
1.734 |
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| 50 |
1.562 |
1.482 |
1.522 |
1.368 |
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| 25 |
1.198 |
1.134 |
1.166 |
1.012 |
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| 12.5 |
0.647 |
0.596 |
0.622 |
0.468 |
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| 6.25 |
0.371 |
0.347 |
0.359 |
0.205 |
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| 3.12 |
0.234 |
0.219 |
0.227 |
0.073 |
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| 0 |
0.158 |
0.149 |
0.154 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
