Human Galectin-3-binding protein(LGALS3BP) ELISA kit
Product Details
| Target Name |
lectin, galactoside-binding, soluble, 3 binding protein |
| Alternative Names |
90K ELISA Kit; 90K MAC 2 BP ELISA Kit; Basement membrane autoantigen p105 ELISA Kit; BTBD17B ELISA Kit; Galectin 3 binding protein ELISA Kit; Galectin-3-binding protein ELISA Kit; L3 antigen ELISA Kit; Lectin galactoside binding soluble 3 binding protein ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; LG3BP_HUMAN ELISA Kit; LGALS3BP ELISA Kit; M2BP ELISA Kit; Mac 2 binding protein ELISA Kit; Mac 2 BP ELISA Kit; Mac-2 BP ELISA Kit; Mac-2-binding protein ELISA Kit; Mac2 BP ELISA Kit; MAC2BP ELISA Kit; Serum protein 90K ELISA Kit; TANGO10B ELISA Kit; Transport and golgi organization 10 homolog B ELISA Kit; Tumor associated antigen 90K ELISA Kit; Tumor-associated antigen 90K ELISA Kit |
| Abbreviation |
LGALS3BP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
91 |
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| Range % |
84-98 |
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| 1:40 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:60 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:160 |
Average % |
102 |
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| Range % |
96-108 |
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| Recovery |
| The recovery of human LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
95-106 |
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| EDTA plasma (n=4) |
90 |
85-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.620 |
2.645 |
2.633 |
2.541 |
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| 50 |
2.265 |
2.366 |
2.316 |
2.224 |
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| 25 |
1.628 |
1.673 |
1.651 |
1.559 |
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| 12.5 |
0.921 |
0.855 |
0.888 |
0.796 |
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| 6.25 |
0.571 |
0.589 |
0.580 |
0.488 |
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| 3.12 |
0.333 |
0.355 |
0.344 |
0.252 |
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| 1.56 |
0.190 |
0.188 |
0.189 |
0.097 |
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| 0 |
0.093 |
0.090 |
0.092 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
