Human gamma glutamyl transpeptidase,GGT ELISA Kit
Product Details
| Target Name |
gamma-glutamyltransferase 1 |
| Alternative Names |
CD224 ELISA Kit; D22S672 ELISA Kit; D22S732 ELISA Kit; Gamma glutamyl transpeptidase ELISA Kit; Gamma glutamyltransferase 1 ELISA Kit; Gamma glutamyltranspeptidase 1 ELISA Kit; Gamma-glutamyltransferase 1 ELISA Kit; Gamma-glutamyltranspeptidase 1 light chain ELISA Kit; GGT 1 ELISA Kit; GGT ELISA Kit; GGT1 ELISA Kit; GGT1_HUMAN ELISA Kit; Glutamyl transpeptidase ELISA Kit; Glutathione hydrolase 1 ELISA Kit; GTG ELISA Kit; Leukotriene C4 hydrolase ELISA Kit; MGC96892 ELISA Kit; MGC96904 ELISA Kit; MGC96963 ELISA Kit; OTTHUMP00000028921 ELISA Kit; OTTHUMP00000197959 ELISA Kit |
| Abbreviation |
GGT1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 mU/mL-50 mU/mL |
| Sensitivity |
0.195 mU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-112 |
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| Recovery |
| The recovery of human GGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-98 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
1.991 |
2.069 |
2.030 |
1.846 |
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| 25 |
1.438 |
1.494 |
1.466 |
1.282 |
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| 12.5 |
0.996 |
1.032 |
1.014 |
0.830 |
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| 6.25 |
0.720 |
0.711 |
0.716 |
0.532 |
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| 3.12 |
0.484 |
0.477 |
0.481 |
0.297 |
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| 1.56 |
0.348 |
0.338 |
0.343 |
0.159 |
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| 0.78 |
0.249 |
0.265 |
0.257 |
0.073 |
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| 0 |
0.181 |
0.186 |
0.184 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
