Human glucagon-like peptide-1,GLP-1 ELISA Kit
Product Details
| Target Name |
glucagon-like peptide-1,GLP-1 |
| Alternative Names |
N/A |
| Abbreviation |
GLP-1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
2.3 ng/mL-40 ng/mL |
| Sensitivity |
1.4 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
87-107 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-106 |
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| 1:4 |
Average % |
95 |
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| Range % |
86-105 |
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| 1:8 |
Average % |
96 |
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| Range % |
89-100 |
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| Recovery |
| The recovery of human GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-101 |
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| EDTA plasma (n=4) |
95 |
89-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 2.3 |
0.063 |
0.061 |
0.062 |
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| 4.6 |
0.169 |
0.172 |
0.171 |
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| 9.1 |
0.398 |
0.384 |
0.391 |
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| 22.9 |
0.861 |
0.872 |
0.867 |
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| 40 |
1.660 |
1.653 |
1.657 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
