Human Glucagon,GC ELISA Kit
Product Details
| Description |
The product CSB-E09207h is a ready-to-use solid-phase ELISA kit for the quantitative determination of Glucagon (GC) levels in human biological fluids, including serum, plasma, and tissue homogenates. Glucagon is a 29 amino acid peptide manufactured by the alpha cells in the pancreatic islets. Glucagon and insulin work synergistically to maintain blood glucose homeostasis. It mainly functions to increase blood glucose through glycogenolysis and gluconeogenesis. Classically hypoglycemia stimulates glucagon release which in turn promotes hepatic gluconeogenesis and glycogenolysis to normalize plasma glucose levels. Excessive glucagon may lead to glucagonoma syndrome, containing weight loss, necrolytic migratory erythema (NME), diabetes, and mucosal abnormalities including stomatitis, cheilitis, and glossitis. Glucagon also affects lipid metabolism, breaking down fat through lipolysis and increasing ketone production. In the protein metabolism, glucagon increases ureagenesis and causes ami... Read more |
| Target Name |
glucagon |
| Alternative Names |
GCG ELISA Kit; Glicentin related polypeptide ELISA Kit; glicentin-related polypeptide ELISA Kit; GLP-1 ELISA Kit; GLP-1(7-36) ELISA Kit; GLP-1(7-37) ELISA Kit; GLP-2 ELISA Kit; GLP1 ELISA Kit; GLP1; included ELISA Kit; GLP2 ELISA Kit; GLP2; included ELISA Kit; GLUC_HUMAN ELISA Kit; Glucagon ELISA Kit; Glucagon like peptide 1 ELISA Kit; glucagon-like peptide 1 ELISA Kit; Glucagon-like peptide 1; included ELISA Kit; Glucagon-like peptide 2 ELISA Kit; Glucagon-like peptide 2; included ELISA Kit; GRPP ELISA Kit; OXM ELISA Kit; OXY ELISA Kit; preproglucagon ELISA Kit |
| Abbreviation |
GCG |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.9 pg/mL-200 pg/mL |
| Sensitivity |
4.3 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-94 |
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| EDTA plasma (n=4) |
99 |
93-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.534 |
2.552 |
2.543 |
2.514 |
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| 100 |
1.312 |
1.276 |
1.294 |
1.265 |
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| 57.2 |
0.642 |
0.614 |
0.628 |
0.599 |
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| 28.6 |
0.271 |
0.259 |
0.265 |
0.236 |
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| 12.9 |
0.122 |
0.124 |
0.123 |
0.094 |
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| 0 |
0.028 |
0.029 |
0.029 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-glucagon antibody.
- Six vials standard (5ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated glucagon antibody (6 ml/bottle) --- Bind to the glucagon in the samples or standards and react with the substrate to make the solution chromogenic.
- One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
- One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
- One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
- One vial Stop Solution (7ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
