Human Glutamate carboxypeptidase 2(FOLH1) ELISA kit
Product Details
| Target Name |
folate hydrolase (prostate-specific membrane antigen) 1 |
| Alternative Names |
Cell growth inhibiting protein 27 ELISA Kit; Cell growth-inhibiting gene 27 protein ELISA Kit; FGCP ELISA Kit; Folate hydrolase (prostate-specific membrane antigen) 1 ELISA Kit; Folate hydrolase 1 ELISA Kit; Folate hydrolase ELISA Kit; Folate hydrolase prostate specific membrane antigen 1 ELISA Kit; FOLH 1 ELISA Kit; FOLH ELISA Kit; Folh1 ELISA Kit; FOLH1_HUMAN ELISA Kit; Folylpoly gamma glutamate carboxypeptidase ELISA Kit; Folylpoly-gamma-glutamate carboxypeptidase ELISA Kit; GCP 2 ELISA Kit; GCP II ELISA Kit; GCP2 ELISA Kit; GCPII ELISA Kit; GIG27 ELISA Kit; Glutamate carboxylase II ELISA Kit; Glutamate carboxypeptidase 2 ELISA Kit; Glutamate carboxypeptidase II ELISA Kit; Membrane glutamate carboxypeptidase ELISA Kit; mGCP ELISA Kit; N acetylated alpha linked acidic dipeptidase 1 ELISA Kit; N-acetylated-alpha-linked acidic dipeptidase I ELISA Kit; NAALAD 1 ELISA Kit; NAALAD1 ELISA Kit; NAALAdase ELISA Kit; NAALADase I ELISA Kit; Prostate specific membrane antigen ELISA Kit; Prostate specific membrane antigen variant F ELISA Kit; Prostate-specific membrane antigen ELISA Kit; PSM ELISA Kit; PSMA ELISA Kit; Pteroylpoly gamma glutamate carboxypeptidase ELISA Kit; Pteroylpoly-gamma-glutamate carboxypeptidase ELISA Kit |
| Abbreviation |
FOLH1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
4.7 ng/mL-300 ng/mL |
| Sensitivity |
1.2 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human FOLH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
92 |
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| Range % |
85-98 |
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| 1:10 |
Average % |
95 |
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| Range % |
85-100 |
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| 1:20 |
Average % |
96 |
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| Range % |
89-103 |
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| 1:40 |
Average % |
89 |
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| Range % |
81-98 |
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| Recovery |
| The recovery of human FOLH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
89-105 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 300 |
2.871 |
2.889 |
2.880 |
2.776 |
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| 150 |
2.529 |
2.532 |
2.531 |
2.427 |
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| 75 |
1.882 |
1.897 |
1.890 |
1.786 |
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| 37.5 |
1.174 |
1.165 |
1.170 |
1.066 |
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| 18.8 |
0.699 |
0.706 |
0.703 |
0.599 |
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| 9.4 |
0.482 |
0.501 |
0.492 |
0.388 |
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| 4.7 |
0.308 |
0.315 |
0.312 |
0.208 |
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| 0 |
0.106 |
0.102 |
0.104 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
