Human glutamic acid decarboxylase,GAD ELISA Kit
Product Details
| Target Name |
glutamate decarboxylase 1 (brain, 67kDa) |
| Alternative Names |
67 kDa glutamic acid decarboxylase ELISA Kit; CPSQ1 ELISA Kit; DCE1 ELISA Kit; DCE1_HUMAN ELISA Kit; EC 4.1.1.15 ELISA Kit; FLJ45882 ELISA Kit; GAD 1 ELISA Kit; GAD 67 ELISA Kit; GAD ELISA Kit; GAD-67 ELISA Kit; GAD1 ELISA Kit; Glutamate decarboxylase 1 (brain; 67kDa) ELISA Kit; Glutamate decarboxylase 1 ELISA Kit; Glutamate decarboxylase 1 brain 67kD ELISA Kit; Glutamate decarboxylase 1 brain 67kDa ELISA Kit; Glutamate decarboxylase 67 kDa isoform ELISA Kit; Glutamate decarboxylase; brain; 67-KD ELISA Kit; OTTHUMP00000041054 ELISA Kit; OTTHUMP00000041055 ELISA Kit; OTTHUMP00000228572 ELISA Kit; SCP ELISA Kit |
| Abbreviation |
GAD1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GAD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
90-98 |
|
| 1:2 |
Average % |
86 |
|
| Range % |
82-93 |
|
| 1:4 |
Average % |
95 |
|
| Range % |
90-100 |
|
| 1:8 |
Average % |
92 |
|
| Range % |
87-96 |
|
|
| Recovery |
| The recovery of human GAD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-95 |
|
| EDTA plasma (n=4) |
99 |
95-102 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.512 |
2.458 |
2.485 |
2.357 |
|
| 10 |
1.678 |
1.788 |
1.733 |
1.605 |
|
| 5 |
1.105 |
1.190 |
1.148 |
1.020 |
|
| 2.5 |
0.685 |
0.710 |
0.698 |
0.570 |
|
| 1.25 |
0.474 |
0.465 |
0.470 |
0.342 |
|
| 0.625 |
0.340 |
0.321 |
0.331 |
0.203 |
|
| 0.312 |
0.240 |
0.221 |
0.231 |
0.103 |
|
| 0 |
0.125 |
0.131 |
0.128 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
