Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2) ELISA kit
Product Details
| Target Name |
glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) |
| Alternative Names |
AATM_HUMAN ELISA Kit; AL022787 ELISA Kit; Aspartate aminotransferase 2 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase; mitochondrial ELISA Kit; Aspartate transaminase 2 ELISA Kit; ASPATA ELISA Kit; EC 2.6.1.1 ELISA Kit; FABP 1 ELISA Kit; FABP pm ELISA Kit; FABP-1 ELISA Kit; FABPpm ELISA Kit; Fatty acid binding protein ELISA Kit; Fatty acid-binding protein ELISA Kit; FLJ40994 ELISA Kit; Glutamate oxaloacetate transaminase 2 ELISA Kit; Glutamate oxaloacetate transaminase 2; mitochondrial ELISA Kit; Glutamate oxaloacetate transaminase; mitochondrial ELISA Kit; Glutamic oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2) ELISA Kit; Got 2 ELISA Kit; GOT2 ELISA Kit; KAT4 ELISA Kit; KATIV ELISA Kit; kynurenine aminotransferase 4 ELISA Kit; Kynurenine aminotransferase IV ELISA Kit; kynurenine--oxoglutarate transaminase 4 ELISA Kit; kynurenine--oxoglutarate transaminase IV ELISA Kit; mAspAT ELISA Kit; MGC102129 ELISA Kit; MGC115763 ELISA Kit; mitAAT ELISA Kit; mitochondrial ELISA Kit; Mitochondrial aspartate aminotransferase ELISA Kit; OTTMUSP00000017748 ELISA Kit; Plasma membrane fatty acid binding protein ELISA Kit; Plasma membrane-associated fatty acid-binding protein ELISA Kit; Transaminase A ELISA Kit |
| Abbreviation |
GOT2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 mU/mL-10 mU/mL |
| Sensitivity |
0.039 mU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GOT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-89 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-97 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-100 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-91 |
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| Recovery |
| The recovery of human GOT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
103 |
98-109 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.186 |
2.268 |
2.227 |
2.028 |
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| 5 |
1.765 |
1.794 |
1.780 |
1.581 |
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| 2.5 |
1.391 |
1.380 |
1.386 |
1.187 |
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| 1.25 |
0.928 |
0.911 |
0.920 |
0.721 |
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| 0.625 |
0.654 |
0.623 |
0.639 |
0.440 |
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| 0.312 |
0.439 |
0.459 |
0.449 |
0.250 |
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| 0.156 |
0.327 |
0.341 |
0.334 |
0.135 |
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| 0 |
0.202 |
0.195 |
0.199 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
