18 years of successful growth

Human Glycodelin ELISA Kit

Product Details

Target Name progestagen-associated endometrial protein
Alternative Names Alpha uterine protein ELISA Kit; gD ELISA Kit; GdA ELISA Kit; GdF ELISA Kit; GdS ELISA Kit; Glycodelin A ELISA Kit; Glycodelin ELISA Kit; Glycodelin F ELISA Kit; Glycodelin S ELISA Kit; MGC138509 ELISA Kit; MGC142288 ELISA Kit; PAEG ELISA Kit; PAEP ELISA Kit; PAEP_HUMAN ELISA Kit; PEG ELISA Kit; PEP ELISA Kit; Placental Protein 14 / Glycodelin A ELISA Kit; Placental protein 14 ELISA Kit; PP14 ELISA Kit; Pregnancy-associated endometrial alpha-2 globulin ELISA Kit; Progestagen-associated endometrial protein ELISA Kit; Progesterone-associated endometrial protein ELISA Kit
Abbreviation PAEP
Species Homo sapiens (Human)
Sample Types serum, cerebrospinal fluid (CSF), urine, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human glycodelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-92  
1:2 Average % 99  
Range % 94-105  
1:4 Average % 103  
Range % 92-110  
1:8 Average % 91  
Range % 86-96  
Recovery
The recovery of human glycodelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 115 110-120  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.821 2.687 2.754 2.631  
25 2.287 2.193 2.240 2.117  
12.5 1.642 1.574 1.608 1.485  
6.25 0.964 0.931 0.948 0.825  
3.12 0.581 0.563 0.572 0.449  
1.56 0.389 0.382 0.386 0.263  
0.78 0.265 0.257 0.261 0.138  
0 0.125 0.121 0.123    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Download X-Ray Brochures