Human Glypican-3,GPC-3 ELISA Kit
Product Details
| Target Name |
glypican 3 |
| Alternative Names |
DGSX ELISA Kit; Glypican proteoglycan 3 ELISA Kit; Glypican-3 [Precursor] ELISA Kit; Gpc3 ELISA Kit; GPC3_HUMAN ELISA Kit; GTR2 2 ELISA Kit; GTR2-2 ELISA Kit; Heparan sulphate proteoglycan ELISA Kit; Intestinal protein OCI 5 ELISA Kit; Intestinal protein OCI-5 ELISA Kit; MXR7 ELISA Kit; OCI 5 ELISA Kit; OCI-5 ELISA Kit; OCI5 ELISA Kit; SDYS ELISA Kit; Secreted glypican-3 ELISA Kit; SGB ELISA Kit; SGBS ELISA Kit; SGBS1 ELISA Kit |
| Abbreviation |
GPC3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, urine, cell culture supernates |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
80-100 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
100 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.621 |
2.641 |
2.631 |
2.437 |
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| 20 |
2.356 |
2.194 |
2.275 |
2.081 |
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| 10 |
1.626 |
1.674 |
1.650 |
1.456 |
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| 5 |
1.021 |
1.101 |
1.061 |
0.867 |
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| 2.5 |
0.596 |
0.600 |
0.598 |
0.404 |
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| 1.25 |
0.412 |
0.422 |
0.417 |
0.223 |
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| 0.625 |
0.302 |
0.315 |
0.309 |
0.115 |
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| 0 |
0.187 |
0.201 |
0.194 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
