Product Details

Description

This Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit is used for the quantitative measurement of human GM-CSF in many biological fluids, including serum, plasma, cell culture supernates, and tissue homogenates. GM-CSF, also known as CSF2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells, and fibroblasts. It is an important cytokine that exerts effects in both innate and adaptive immunity. Acting as an inducer, CSF2 stimulates myeloid progenitors to proliferate and differentiate into granulocyte and macrophage. CSF2 binding to its receptor CSF2R triggers four main signaling pathways, including JAK/STAT, PI3K-AKT, ERK1/2, and NF-kB pathways, thus promoting cell differentiation, survival, and inflammation. The assay is based on the Sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The intensity of the color is proportional to the GM-CSF content in the sample. This kit has been veri...Read more

Target Name

colony stimulating factor 2 (granulocyte-macrophage)

Alternative Names

Colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit; Colony Stimulating Factor 2 ELISA Kit; Colony stimulating factor ELISA Kit; Colony-stimulating factor ELISA Kit; CSF 2 ELISA Kit; CSF ELISA Kit; CSF2 ELISA Kit; CSF2_HUMAN ELISA Kit; GM-CSF ELISA Kit; GMCSF ELISA Kit; Granulocyte Macrophage Colony Stimulating Factor ELISA Kit; Granulocyte-macrophage colony-stimulating factor ELISA Kit; MGC131935 ELISA Kit; MGC138897 ELISA Kit; MGI1GM ELISA Kit; Molgramostin ELISA Kit; Pluripoietin-a ELISA Kit; Sargramostim ELISA Kit

Abbreviation

CSF2

Uniprot No.

P04141

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

• Intra-assay Precision (Precision within an assay): CV%
• Three samples of known concentration were tested twenty times on one plate to assess. 
• Inter-assay Precision (Precision between assays): CV%
• Three samples of known concentration were tested in twenty assays to assess.