Human Growth arrest-specific protein 6(GAS6) ELISA kit
Product Details
| Target Name |
growth arrest-specific 6 |
| Alternative Names |
AXL receptor tyrosine kinase ligand ELISA Kit; AXL stimulatory factor ELISA Kit; AXLLG ELISA Kit; AXS F ELISA Kit; AXSF ELISA Kit; DKFZp666G247 ELISA Kit; FLJ34709 ELISA Kit; Gas 6 ELISA Kit; GAS-6 ELISA Kit; Gas6 ELISA Kit; GAS6_HUMAN ELISA Kit; Growth arrest specific 6 ELISA Kit; Growth arrest-specific protein 6 ELISA Kit; Growth-arrest-specific protein 6 ELISA Kit; Growth-arrest-specific protein 6 precursor ELISA Kit |
| Abbreviation |
GAS6 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GAS6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
89-96 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-92 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-102 |
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| Recovery |
| The recovery of human GAS6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
92-97 |
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| EDTA plasma (n=4) |
96 |
92-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.388 |
2.352 |
2.370 |
2.226 |
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| 25 |
1.662 |
1.576 |
1.619 |
1.475 |
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| 12.5 |
1.061 |
1.042 |
1.052 |
0.908 |
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| 6.25 |
0.614 |
0.600 |
0.607 |
0.463 |
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| 3.12 |
0.409 |
0.417 |
0.413 |
0.269 |
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| 1.56 |
0.303 |
0.309 |
0.306 |
0.162 |
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| 0.78 |
0.238 |
0.241 |
0.240 |
0.096 |
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| 0 |
0.142 |
0.146 |
0.144 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
