Human Heat Shock Protein 72(HSP-72) ELISA Kit
Product Details
| Target Name |
Heat Shock Protein 72(HSP-72) |
| Alternative Names |
HSPA1A ELISA Kit; HSP72 ELISA Kit; HSPA1 ELISA Kit; HSX70Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit; HSP70.1 ELISA Kit |
| Abbreviation |
HSP72 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, cell lysates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
101 |
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| Range % |
92-108 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-96 |
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| Recovery |
| The recovery of human HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-98 |
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| EDTA plasma (n=4) |
99 |
92-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.868 |
2.856 |
2.862 |
2.706 |
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| 100 |
2.436 |
2.387 |
2.412 |
2.256 |
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| 50 |
1.694 |
1.668 |
1.681 |
1.525 |
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| 25 |
1.034 |
1.024 |
1.029 |
0.873 |
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| 12.5 |
0.594 |
0.587 |
0.591 |
0.435 |
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| 6.25 |
0.415 |
0.402 |
0.409 |
0.253 |
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| 3.12 |
0.283 |
0.273 |
0.278 |
0.122 |
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| 0 |
0.158 |
0.154 |
0.156 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
