Human heme oxygenase 1,HO-1 ELISA Kit
Product Details
| Target Name |
heme oxygenase (decycling) 1 |
| Alternative Names |
32 kD ELISA Kit; bK286B10 ELISA Kit; D8Wsu38e ELISA Kit; heat shock protein 32 kD ELISA Kit; heat shock protein 32kD ELISA Kit; Heat shock protein ELISA Kit; Heme oxygenase (decycling) 1 ELISA Kit; Heme oxygenase 1 ELISA Kit; Hemox ELISA Kit; HMOX 1 ELISA Kit; Hmox ELISA Kit; Hmox1 ELISA Kit; HMOX1_HUMAN ELISA Kit; HO 1 ELISA Kit; HO ELISA Kit; HO-1 ELISA Kit; HO1 ELISA Kit; Hsp32 ELISA Kit |
| Abbreviation |
HMOX1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 ng/mL-1000 ng/mL |
| Sensitivity |
3.9 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-94 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-105 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-99 |
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| Recovery |
| The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-92 |
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| EDTA plasma (n=4) |
98 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.449 |
2.284 |
2.367 |
2.263 |
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| 500 |
1.967 |
1.864 |
1.916 |
1.812 |
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| 250 |
1.277 |
1.233 |
1.255 |
1.151 |
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| 125 |
0.701 |
0.674 |
0.688 |
0.584 |
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| 62.5 |
0.430 |
0.421 |
0.426 |
0.322 |
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| 31.2 |
0.258 |
0.253 |
0.256 |
0.152 |
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| 15.6 |
0.187 |
0.184 |
0.186 |
0.082 |
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| 0 |
0.104 |
0.103 |
0.104 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
