Human heparan sulfate proteoglycan 2 (HSPG2) ELISA kit
Product Details
| Target Name |
heparan sulfate proteoglycan 2 |
| Alternative Names |
Basement membrane specific heparan sulfate proteoglycan core protein ELISA Kit; Endorepellin (domain V region) ELISA Kit; Heparan sulfate proteoglycan of basement membrane ELISA Kit; HSPG 2 ELISA Kit; HSPG ELISA Kit; Hspg2 ELISA Kit; LG3 peptide ELISA Kit; Perlecan ELISA Kit; PGBM_HUMAN ELISA Kit; PLC ELISA Kit; Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) ELISA Kit; SJA ELISA Kit; SJS ELISA Kit; SJS1 ELISA Kit |
| Abbreviation |
HSPG2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSPG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
102 |
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| Range % |
89-107 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-102 |
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| 1:4 |
Average % |
94 |
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| Range % |
84-98 |
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| 1:8 |
Average % |
106 |
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| Range % |
96-110 |
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| Recovery |
| The recovery of human HSPG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
103 |
93-106 |
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| EDTA plasma (n=4) |
103 |
94-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.171 |
2.089 |
2.130 |
1.978 |
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| 2000 |
1.530 |
1.483 |
1.507 |
1.355 |
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| 1000 |
0.925 |
0.946 |
0.936 |
0.784 |
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| 500 |
0.578 |
0.565 |
0.572 |
0.420 |
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| 250 |
0.352 |
0.367 |
0.360 |
0.208 |
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| 125 |
0.245 |
0.253 |
0.249 |
0.097 |
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| 62.5 |
0.203 |
0.208 |
0.206 |
0.054 |
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| 0 |
0.154 |
0.150 |
0.152 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
