The Human heparan sulfate (HS) ELISA Kit is applied to detect and quantify the concentrations of HS in serum, plasma, urine, and tissue homogenates. This kit exclusively recognizes human HS protein. This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with an HS antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for HS and HRP-conjugated goat-anti-mouse HS antibody. The competitive inhibition reaction is launched between pre-coated HS and HS in samples or samples. A substrate solution is added to the wells and The intensity of the color is negatively relevant to the levels of HS in the sample. The color development is stopped and the intensity of the color is measured.

This ELISA kit has been confirmed to have high sensitivity, excellent specificity, premium precision, and lot-to-lot consistency. See the product instructions for more details.

Heparan sulfate (HS) is a highly negatively charged linear polysaccharide and commonly binds to a small set of the membrane or extracellular matrix proteoglycansa to form heparan sulfate proteoglycans (HSPGs). It is a ubiquitous compound of animal cells. Both HS and HSPGs binds to protein ligands and act as co-receptors for different cell surface receptors, regulating a broad range of biological activities, such as developmental processes, angiogenesis, cell adhesion, immune cell infiltration, blood coagulation, abolishing detachment activity by GrB (Granzyme B), and tumor metastasis. Evidence has demonstrated that HS is deregulated in a variety of solid tumors and hematological malignancies and modulate key aspects of cancer initiation and progression. Additionally, numerous viruses (including emerging SARS-CoV-2) and parasites use cell surface heparan sulfate as receptors to infect target cells.