Human Heparanase,HPA ELISA kit
Product Details
| Target Name |
heparanase |
| Alternative Names |
Endo glucoronidase ELISA Kit; Endo-glucoronidase ELISA Kit; HEP ELISA Kit; Heparanase 50 kDa subunit ELISA Kit; Heparanase ELISA Kit; Heparanase-1 ELISA Kit; Heparanase1 ELISA Kit; Hpa 1 ELISA Kit; HPA ELISA Kit; Hpa1 ELISA Kit; HPR 1 ELISA Kit; HPR1 ELISA Kit; HPSE 1 ELISA Kit; HPSE ELISA Kit; HPSE_HUMAN ELISA Kit; HPSE1 ELISA Kit; HSE 1 ELISA Kit; HSE1 ELISA Kit |
| Abbreviation |
HPSE |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, ascitic fluid, cell culture supernates, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-98 |
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| 1:2 |
Average % |
97 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human HPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
89-110 |
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| EDTA plasma (n=4) |
95 |
90-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.761 |
2.743 |
2.752 |
2.636 |
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| 5 |
2.436 |
2.424 |
2.430 |
2.314 |
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| 2.5 |
1.902 |
1.915 |
1.909 |
1.793 |
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| 1.25 |
1.203 |
1.219 |
1.211 |
1.095 |
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| 0.625 |
0.604 |
0.612 |
0.608 |
0.492 |
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| 0.312 |
0.325 |
0.320 |
0.323 |
0.207 |
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| 0.156 |
0.223 |
0.231 |
0.227 |
0.111 |
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| 0 |
0.112 |
0.119 |
0.116 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
