Product Details

Description

The Human hepatitis B virus e antigen (HBeAg) ELISA kit is used for qualitative identification of HBeAg in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HBeAb. Samples or standards are pipetted into the wells with HRP-conjugated HBeAb. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of HBeAg bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. It indicates the presence of HBeAg if the O.D. (optical density) of HBeAg is greater than or equal to the cutoff value (2.1× Average O.D. value of negative control). There is no HBeAg present in the sample if the O.D. is less than the cutoff value. This assay has high sensitivity and excellent specificity for detection of HBeAg. And it also has been validated with precision less than 20% an...Read more

Target Name

hepatitis B virus e antigen (HBeAg)

Abbreviation

HBeAg

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

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Sensitivity

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Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Microbiology

Assay Principle

qualitative

Measurement

Sandwich

Precision

• Intra-assay Precision (Precision within an assay): CV%
• Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision (Precision between assays): CV%
• Three samples of known concentration were tested in twenty assays to assess.