Product Details

Description

The Human hepatitis D virus (HDV) IgG ELISA kit is used for qualitative identification of IgG antibodies to HDV in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HDV antigen. Samples or standards are pipetted into the wells with anti-human IgG conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human HDV IgG antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The valence of human HDV IgG antibody in the samples is determined by referring to the negative control. It indicates the presence of HDV IgG antibody if the O.D. (optical density) of sample is greater than or equal to the cutoff value (average Negative Control value plus 0.10). There is no HDV IgG antibody present in the sample if the O.D. is less th...Read more

Target Name

hepatitis D virus(HDV) antibody(IgG)

Alternative Names

N/A

Abbreviation

HDV Ab (IgG)

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

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Sensitivity

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Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Microbiology

Precision

• Intra-assay Precision (Precision within an assay): CV%
• Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision (Precision between assays): CV%
• Three samples of known concentration were tested in twenty assays to assess