Human High Molecular Weight Adiponectin(HMW Adiponectin)ELISA Kit
Product Details
| Target Name |
High Molecular Weight Adiponectin(HMW Adiponectin) |
| Alternative Names |
30 kDa adipocyte complement related protein ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; ACDC ELISA Kit; Acrp 30 ELISA Kit; ACRP30 ELISA Kit; ADIPO_HUMAN ELISA Kit; Adipocyte ELISA Kit; Adipocyte C1q and collagen domain containing protein ELISA Kit; Adipocyte complement related 30 kDa protein ELISA Kit; Adipocyte complement related protein of 30 kDa ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; adipocyte-specific secretory protein ELISA Kit; Adiponectin ELISA Kit; Adiponectin precursor ELISA Kit; adiponectin; C1Q and collagen domain containing ELISA Kit; Adipoq ELISA Kit; Adipose most abundant gene transcript 1 ELISA Kit; Adipose most abundant gene transcript 1 protein ELISA Kit; Adipose specific collagen like factor ELISA Kit; ADIPQTL1 ELISA Kit; ADPN ELISA Kit; APM 1 ELISA Kit; apM-1 ELISA Kit; APM1 ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; GBP 28 ELISA Kit; GBP28 ELISA Kit; Gelatin binding protein ELISA Kit; Gelatin binding protein 28 ELISA Kit; gelatin-binding protein 28 ELISA Kit; Gelatin-binding protein ELISA Kit; OTTHUMP00000210047 ELISA Kit |
| Abbreviation |
HMW Adiponectin |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, urine |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HMW adiponectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
87 |
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| Range % |
84-91 |
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| 1:200 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:400 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:800 |
Average % |
101 |
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| Range % |
96-106 |
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| Recovery |
| The recovery of human HMW adiponectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
95-104 |
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| EDTA plasma (n=4) |
92 |
87-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.169 |
2.117 |
2.143 |
2.040 |
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| 100 |
1.679 |
1.658 |
1.669 |
1.566 |
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| 50 |
1.245 |
1.231 |
1.238 |
1.135 |
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| 25 |
0.832 |
0.869 |
0.851 |
0.748 |
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| 12.5 |
0.513 |
0.547 |
0.530 |
0.427 |
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| 6.25 |
0.345 |
0.367 |
0.356 |
0.253 |
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| 3.12 |
0.203 |
0.212 |
0.208 |
0.105 |
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| 0 |
0.102 |
0.104 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
