Human hypoxia-inducible factor 2(HIF-2) ELISA kit
Product Details
| Target Name |
endothelial PAS domain protein 1 |
| Alternative Names |
Basic helix loop helix PAS protein MOP2 ELISA Kit; Basic-helix-loop-helix-PAS protein MOP2 ELISA Kit; bHLHe73 ELISA Kit; Class E basic helix-loop-helix protein 73 ELISA Kit; ECYT4 ELISA Kit; Endothelial PAS domain containing protein 1 ELISA Kit; Endothelial pas domain protein 1 ELISA Kit; Endothelial PAS domain-containing protein 1 ELISA Kit; EPAS 1 ELISA Kit; EPAS-1 ELISA Kit; EPAS1 ELISA Kit; EPAS1_HUMAN ELISA Kit; HIF 1 alpha like factor ELISA Kit; HIF 2 alpha ELISA Kit; HIF-1-alpha-like factor ELISA Kit; HIF-2-alpha ELISA Kit; HIF2-alpha ELISA Kit; HIF2A ELISA Kit; HLF ELISA Kit; Hypoxia inducible factor 2 alpha ELISA Kit; Hypoxia inducible factor 2 alpha subunit ELISA Kit; Hypoxia-inducible factor 2-alpha ELISA Kit; Member of PAS protein 2 ELISA Kit; Member of pas superfamily 2 ELISA Kit; MOP 2 ELISA Kit; MOP2 ELISA Kit; PAS domain-containing protein 2 ELISA Kit; PASD2 ELISA Kit |
| Abbreviation |
EPAS1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HIF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:8 |
Average % |
85 |
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| Range % |
82-93 |
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| Recovery |
| The recovery of human HIF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
87-95 |
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| EDTA plasma (n=4) |
101 |
96-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.760 |
2.740 |
2.750 |
2.605 |
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| 2000 |
2.184 |
2.350 |
2.267 |
2.122 |
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| 1000 |
1.439 |
1.520 |
1.480 |
1.335 |
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| 500 |
0.712 |
0.730 |
0.721 |
0.576 |
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| 250 |
0.445 |
0.431 |
0.438 |
0.293 |
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| 125 |
0.352 |
0.342 |
0.347 |
0.202 |
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| 62.5 |
0.251 |
0.265 |
0.258 |
0.113 |
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| 0 |
0.144 |
0.145 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
