Human indoleamine 2,3-dioxygenase,IDO ELISA Kit
Product Details
| Target Name |
indoleamine 2,3-dioxygenase 1 |
| Alternative Names |
3-dioxygenase ELISA Kit; I23O1_HUMAN ELISA Kit; IDO 1 ELISA Kit; IDO ELISA Kit; IDO-1 ELISA Kit; IDO1 ELISA Kit; INDO ELISA Kit; indolamine 2,3 dioxygenase ELISA Kit; Indole 2 3 dioxygenase ELISA Kit; indoleamine 2 3 dioxygenase 1 ELISA Kit; indoleamine 2 3 dioxygenase ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; Indoleamine pyrrole 2 3 dioxygenase ELISA Kit; Indoleamine-pyrrole 2 ELISA Kit |
| Abbreviation |
IDO1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-102 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-94 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-98 |
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| Recovery |
| The recovery of human IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
91-104 |
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| EDTA plasma (n=4) |
86 |
80-90 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.574 |
2.552 |
2.563 |
2.461 |
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| 25 |
2.078 |
1.949 |
2.014 |
1.912 |
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| 12.5 |
1.228 |
1.278 |
1.253 |
1.151 |
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| 6.25 |
0.537 |
0.560 |
0.549 |
0.447 |
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| 3.12 |
0.326 |
0.340 |
0.333 |
0.231 |
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| 1.56 |
0.204 |
0.197 |
0.201 |
0.099 |
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| 0.78 |
0.159 |
0.152 |
0.156 |
0.054 |
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| 0 |
0.103 |
0.100 |
0.102 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
