| Description |
The Human inducible nitric oxide synthase (iNOS/NOS2) ELISA Kit is used for the quantitative analysis of NOS2 in human serum, plasma, or tissue homogenates. This kit has undergone rigorous quality control in many parameters, including sensitivity, specificity, precision, linearity, recovery, and consistency between batches. The detection mechanism of this assay is based on the Sandwich-ELISA mechanism. NOS2 in the samples or standards are sandwiched between pre-coated NOS2 antibody and Biotin-conjugated NOS2 antibody. The NOS2/pre-coated NOS2 antibody/Biotin-conjugated NOS2P antibody complex is labeled by HRP and then occurs a chromogenic reaction after the addition of the TMB solution. The color intensity is positively related to the NOS2 in the samples. NOS2, one isoform of NOS, is expressed in various cell types including macrophages, dendritic cells, and neutrophils in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. Immunostimu... Read more |
| Target Name |
nitric oxide synthase 2, inducible |
| Alternative Names |
HEP-NOS ELISA Kit; Hepatocyte NOS ELISA Kit; HEPNOS ELISA Kit; inducible ELISA Kit; Inducible nitric oxide synthase ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; MAC NOS ELISA Kit; Macrophage NOS ELISA Kit; Nitric oxide synthase 2 inducible ELISA Kit; Nitric oxide synthase 2 inducible macrophage ELISA Kit; nitric oxide synthase 2A (inducible, hepatocytes) ELISA Kit; Nitric oxide synthase ELISA Kit; Nitric oxide synthase inducible ELISA Kit; nitric oxide synthase, macrophage ELISA Kit; NOS 2 ELISA Kit; NOS ELISA Kit; Nos II ELISA Kit; NOS type II ELISA Kit; nos2 ELISA Kit; NOS2_HUMAN ELISA Kit; NOS2A ELISA Kit; NOS2A, Inducible, Hepatocyte ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit |
| Abbreviation |
NOS2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.9 IU/mL-60 IU/mL |
| Sensitivity |
0.225 IU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
|
| Range % |
89-95 |
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| 1:2 |
Average % |
103 |
|
| Range % |
99-105 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
95-101 |
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| Recovery |
| The recovery of human iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
92-97 |
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| EDTA plasma (n=4) |
96 |
92-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| IU/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 60 |
2.519 |
2.499 |
2.509 |
2.378 |
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| 30 |
1.934 |
1.876 |
1.905 |
1.774 |
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| 15 |
1.277 |
1.281 |
1.279 |
1.148 |
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| 7.5 |
0.695 |
0.683 |
0.689 |
0.558 |
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| 3.75 |
0.442 |
0.434 |
0.438 |
0.307 |
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| 1.8 |
0.317 |
0.302 |
0.310 |
0.179 |
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| 0.9 |
0.218 |
0.225 |
0.222 |
0.091 |
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| 0 |
0.133 |
0.129 |
0.131 |
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| Materials provided |
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human NOS2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled NOS2 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
