Human inhibin B,INH-B ELISA Kit
Product Details
| Target Name |
inhibin B,INH-B |
| Alternative Names |
N/A |
| Abbreviation |
INH-B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
4 pg/mL-800 pg/mL |
| Sensitivity |
2 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:4 |
Average % |
99 |
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| Range % |
96-101 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-96 |
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| Recovery |
| The recovery of human INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-88 |
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| EDTA plasma (n=4) |
96 |
92-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.223 |
0.224 |
0.224 |
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| 200 |
0.355 |
0.362 |
0.359 |
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| 60 |
0.556 |
0.568 |
0.562 |
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| 16 |
0.817 |
0.803 |
0.810 |
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| 4 |
1.374 |
1.275 |
1.325 |
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| 0 |
2.508 |
2.404 |
2.456 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
