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Human Inhibitor of growth protein 4(ING4) ELISA kit

Product Details

Target Name inhibitor of growth family, member 4
Alternative Names Brain my036 protein ELISA Kit; Candidate tumor suppressor p33 ING 1 homolog ELISA Kit; Candidate tumor suppressor p33 ING1 homolog ELISA Kit; D6Wsu147e ELISA Kit; D6Xrf92 ELISA Kit; ING 1 like protein ELISA Kit; ING 4 ELISA Kit; ING1 like protein ELISA Kit; ING4 ELISA Kit; ING4_HUMAN ELISA Kit; Inhibitor of growth family member 4 ELISA Kit; Inhibitor of growth family member 4 long isoform ELISA Kit; Inhibitor of growth protein 4 ELISA Kit; MGC12557 ELISA Kit; my036 ELISA Kit; p29 ING 4 ELISA Kit; p29 ING4 ELISA Kit; p29ING4 ELISA Kit
Abbreviation ING4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ING4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-92  
1:2 Average % 101  
Range % 97-103  
1:4 Average % 96  
Range % 92-99  
1:8 Average % 91  
Range % 87-95  
Recovery
The recovery of human ING4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-93  
EDTA plasma (n=4) 104 99-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.714 2.642 2.678 2.565  
1500 1.538 1.544 1.541 1.428  
750 0.749 0.788 0.769 0.656  
375 0.491 0.500 0.496 0.383  
187.5 0.301 0.312 0.307 0.194  
94 0.224 0.232 0.228 0.115  
47 0.183 0.174 0.179 0.066  
0 0.114 0.112 0.113    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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