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Human insulin-like growth factors 2 receptor,IGF-2R ELISA Kit

Product Details

Target Name insulin-like growth factor 2 receptor
Alternative Names 300 kDa mannose 6 phosphate receptor ELISA Kit; 300 kDa mannose 6-phosphate receptor ELISA Kit; Cation independent mannose 6 phosphate receptor ELISA Kit; Cation-independent mannose-6-phosphate receptor ELISA Kit; CD222 ELISA Kit; CD222 antigen ELISA Kit; CI Man 6 P receptor ELISA Kit; CI Man-6-P receptor ELISA Kit; CI MPR ELISA Kit; CI-M6PR ELISA Kit; CI-MPR ELISA Kit; CIMPR ELISA Kit; IGF 2 receptor ELISA Kit; IGF 2R ELISA Kit; IGF II receptor ELISA Kit; IGF-II receptor ELISA Kit; IGF2 receptor ELISA Kit; Igf2r ELISA Kit; Insulin like growth factor 2 receptor ELISA Kit; Insulin like growth factor II receptor ELISA Kit; Insulin-like growth factor 2 receptor ELISA Kit; Insulin-like growth factor II receptor ELISA Kit; M6P R ELISA Kit; M6P/IGF2 receptor ELISA Kit; M6P/IGF2R ELISA Kit; M6PR ELISA Kit; mannose 6 phosphate receptor ELISA Kit; mannose 6 phosphate receptor, cation independent ELISA Kit; MPR 300 ELISA Kit; MPR300 ELISA Kit; MPRI ELISA Kit; MPRI_HUMAN ELISA Kit
Abbreviation IGF2R
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 312.5 pg/mL-20000 pg/mL
Sensitivity 78.1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8>      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10>      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-103  
1:2 Average % 90  
Range % 83-96  
1:4 Average % 102  
Range % 99-105  
1:8 Average % 86  
Range % 81-92  
Recovery
The recovery of human IGF-2R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-98  
EDTA plasma (n=4) 89 82-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
20000 2.821 2.752 2.787 2.666  
10000 2.420 2.279 2.350 2.229  
5000 1.764 1.789 1.777 1.656  
2500 0.941 1.002 0.972 0.851  
1250 0.527 0.555 0.541 0.420  
625 0.318 0.346 0.332 0.211  
312.5 0.214 0.225 0.220 0.099  
0 0.126 0.116 0.121    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days