Human insulin-like growth factors 2 receptor,IGF-2R ELISA Kit
Product Details
| Target Name |
insulin-like growth factor 2 receptor |
| Alternative Names |
300 kDa mannose 6 phosphate receptor ELISA Kit; 300 kDa mannose 6-phosphate receptor ELISA Kit; Cation independent mannose 6 phosphate receptor ELISA Kit; Cation-independent mannose-6-phosphate receptor ELISA Kit; CD222 ELISA Kit; CD222 antigen ELISA Kit; CI Man 6 P receptor ELISA Kit; CI Man-6-P receptor ELISA Kit; CI MPR ELISA Kit; CI-M6PR ELISA Kit; CI-MPR ELISA Kit; CIMPR ELISA Kit; IGF 2 receptor ELISA Kit; IGF 2R ELISA Kit; IGF II receptor ELISA Kit; IGF-II receptor ELISA Kit; IGF2 receptor ELISA Kit; Igf2r ELISA Kit; Insulin like growth factor 2 receptor ELISA Kit; Insulin like growth factor II receptor ELISA Kit; Insulin-like growth factor 2 receptor ELISA Kit; Insulin-like growth factor II receptor ELISA Kit; M6P R ELISA Kit; M6P/IGF2 receptor ELISA Kit; M6P/IGF2R ELISA Kit; M6PR ELISA Kit; mannose 6 phosphate receptor ELISA Kit; mannose 6 phosphate receptor, cation independent ELISA Kit; MPR 300 ELISA Kit; MPR300 ELISA Kit; MPRI ELISA Kit; MPRI_HUMAN ELISA Kit |
| Abbreviation |
IGF2R |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
312.5 pg/mL-20000 pg/mL |
| Sensitivity |
78.1 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
| |
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10>
| |
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
97 |
|
| Range % |
92-103 |
|
| 1:2 |
Average % |
90 |
|
| Range % |
83-96 |
|
| 1:4 |
Average % |
102 |
|
| Range % |
99-105 |
|
| 1:8 |
Average % |
86 |
|
| Range % |
81-92 |
|
|
| Recovery |
| The recovery of human IGF-2R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-98 |
|
| EDTA plasma (n=4) |
89 |
82-95 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20000 |
2.821 |
2.752 |
2.787 |
2.666 |
|
| 10000 |
2.420 |
2.279 |
2.350 |
2.229 |
|
| 5000 |
1.764 |
1.789 |
1.777 |
1.656 |
|
| 2500 |
0.941 |
1.002 |
0.972 |
0.851 |
|
| 1250 |
0.527 |
0.555 |
0.541 |
0.420 |
|
| 625 |
0.318 |
0.346 |
0.332 |
0.211 |
|
| 312.5 |
0.214 |
0.225 |
0.220 |
0.099 |
|
| 0 |
0.126 |
0.116 |
0.121 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |