Human insulin-like growth factors binding protein 2,IGFBP-2 ELISA Kit
Product Details
| Target Name |
insulin-like growth factor binding protein 2, 36kDa |
| Alternative Names |
BP 2 ELISA Kit; BP2 ELISA Kit; IBP 2 ELISA Kit; IBP-2 ELISA Kit; IBP2 ELISA Kit; IBP2_HUMAN ELISA Kit; IGF binding protein 2 ELISA Kit; IGF BP53 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP 2 ELISA Kit; IGFBP-2 ELISA Kit; IGFBP2 ELISA Kit; IGFBP53 ELISA Kit; Insulin like growth factor binding protein 2 36kDa ELISA Kit; Insulin like growth factor binding protein 2 ELISA Kit; Insulin like growth factor-binding protein 2 precursor ELISA Kit; Insulin-like growth factor-binding protein 2 ELISA Kit |
| Abbreviation |
IGFBP2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:10 |
Average % |
95 |
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| Range % |
92-98 |
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| 1:20 |
Average % |
92 |
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| Range % |
86-99 |
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| 1:40 |
Average % |
94 |
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| Range % |
89-100 |
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| Recovery |
| The recovery of human IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
89-101 |
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| EDTA plasma (n=4) |
94 |
88-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.935 |
2.952 |
2.944 |
2.767 |
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| 25 |
2.026 |
2.076 |
2.051 |
1.874 |
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| 12.5 |
1.088 |
1.046 |
1.067 |
0.890 |
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| 6.25 |
0.592 |
0.583 |
0.588 |
0.411 |
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| 3.12 |
0.361 |
0.337 |
0.349 |
0.172 |
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| 1.56 |
0.278 |
0.259 |
0.269 |
0.092 |
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| 0.78 |
0.232 |
0.229 |
0.231 |
0.054 |
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| 0 |
0.176 |
0.177 |
0.177 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
