Human Integrin alpha-2(ITGA2) ELISA kit
Product Details
| Target Name |
integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) |
| Alternative Names |
BR ELISA Kit; CD 49b ELISA Kit; CD49 antigen like family member B ELISA Kit; CD49 antigen-like family member B ELISA Kit; CD49b ELISA Kit; CD49b antigen ELISA Kit; Collagen receptor ELISA Kit; DX5 ELISA Kit; Glycoprotein Ia deficiency included ELISA Kit; GP Ia ELISA Kit; GP Ia deficiency; included ELISA Kit; GPIa ELISA Kit; HPA 5 included ELISA Kit; HPA5 included ELISA Kit; Human platelet alloantigen system 5 ELISA Kit; Integrin alpha 2 ELISA Kit; Integrin alpha II ELISA Kit; Integrin alpha-2 ELISA Kit; Integrin; alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) ELISA Kit; ITA2_HUMAN ELISA Kit; ITGA2 ELISA Kit; Platelet alloantigen Br(a); included ELISA Kit; Platelet antigen Br ELISA Kit; Platelet glycoprotein GPIa ELISA Kit; Platelet glycoprotein Ia ELISA Kit; Platelet glycoprotein Ia/IIa ELISA Kit; Platelet membrane glycoprotein Ia ELISA Kit; Platelet receptor for collagen; deficiency of; included ELISA Kit; Very late activation protein 2 receptor alpha 2 subunit ELISA Kit; VLA 2 alpha chain ELISA Kit; VLA 2 ELISA Kit; VLA 2 subunit alpha ELISA Kit; VLA-2 subunit alpha ELISA Kit; VLA2 ELISA Kit; VLA2 receptor alpha 2 subunit ELISA Kit; VLAA2 ELISA Kit |
| Abbreviation |
ITGA2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.235 ng/mL-15 ng/mL |
| Sensitivity |
0.058 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:10 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:20 |
Average % |
92 |
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| Range % |
89-96 |
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| 1:40 |
Average % |
95 |
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| Range % |
93-97 |
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| Recovery |
| The recovery of human ITGA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
93-104 |
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| EDTA plasma (n=4) |
93 |
90-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 15 |
2.492 |
2.544 |
2.518 |
2.339 |
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| 7.5 |
2.150 |
2.231 |
2.191 |
2.012 |
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| 3.75 |
1.567 |
1.625 |
1.596 |
1.417 |
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| 1.88 |
0.944 |
0.955 |
0.950 |
0.771 |
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| 0.94 |
0.548 |
0.587 |
0.568 |
0.389 |
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| 0.47 |
0.353 |
0.352 |
0.353 |
0.174 |
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| 0.235 |
0.254 |
0.266 |
0.260 |
0.081 |
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| 0 |
0.177 |
0.180 |
0.179 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
