Human Integrin beta-3(ITGB3) ELISA kit
Product Details
| Target Name |
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| Alternative Names |
BDPLT16 ELISA Kit; BDPLT2 ELISA Kit; CD 61 ELISA Kit; CD61 ELISA Kit; CD61 antigen ELISA Kit; GP3A ELISA Kit; GPIIIa ELISA Kit; GT ELISA Kit; HPA 1 ELISA Kit; HPA 4 ELISA Kit; Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) ELISA Kit; Integrin beta chain beta 3 ELISA Kit; Integrin beta-3 ELISA Kit; ITB3_HUMAN ELISA Kit; ITG B3 ELISA Kit; ITGB 3 ELISA Kit; ITGB3 ELISA Kit; NAIT ELISA Kit; platelet fibrinogen receptor ELISA Kit; Platelet fibrinogen receptor beta subunit ELISA Kit; Platelet fibrinogen receptor, beta subunit ELISA Kit; Platelet glycoprotein IIIa ELISA Kit; Platelet glycoprotein IIIa precursor ELISA Kit; Platelet membrane glycoprotein IIIa ELISA Kit; PTP ELISA Kit |
| Abbreviation |
ITGB3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
125 pg/mL-8000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-96 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
103 |
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| Range % |
97-108 |
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| 1:8 |
Average % |
89 |
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| Range % |
85-94 |
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| Recovery |
| The recovery of human ITGB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-97 |
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| EDTA plasma (n=4) |
97 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 8000 |
2.433 |
2.579 |
2.506 |
2.362 |
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| 4000 |
1.887 |
1.936 |
1.912 |
1.768 |
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| 2000 |
1.406 |
1.488 |
1.447 |
1.303 |
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| 1000 |
0.949 |
1.015 |
0.982 |
0.838 |
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| 500 |
0.635 |
0.669 |
0.652 |
0.508 |
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| 250 |
0.371 |
0.345 |
0.358 |
0.214 |
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| 125 |
0.262 |
0.247 |
0.255 |
0.111 |
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| 0 |
0.140 |
0.148 |
0.144 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
