Product Details

Description

CUSABIO’s Human intercellular adhesion molecule 1 (ICAM1) ELISA Kit is specifically designed to standardize the detection of ICAM1 in human serum, plasma, or tissue homogenates. ICAM1 is a transmembrane glycoprotein of the immunoglobulin (Ig)-like superfamily and is expressed by several cell types such as fibroblasts, endothelial, and leucocytes. It exerts important effects in cell-cell adhesion, cell-extracellular matrix (ECM) interaction, transendothelial migration of leukocytes to sites of inflammation, and activation of lymphocytes by interacting with leucocyte function-associated antigen (LFA-1). Increased levels of ICAM1 have been reported in some types of cancer and are related to a more favorable prognosis in gastric, breast, and colorectal cancers. ICAM1 plays a vital role in antitumor immunity through its involvement in T cell priming by antigen-presenting cells, transendothelial trafficking of effector cells, and promoting lymphocyte adhesion with tumor cells. Whereas, ICAM1...Read more

Alternative Names

Antigen identified by monoclonal ELISA Kit BB2 ELISA Kit; BB 2 ELISA Kit; BB2 ELISA Kit; CD 54 ELISA Kit; CD_antigen=CD54 ELISA Kit; CD54 ELISA Kit; Cell surface glycoprotein P3.58 ELISA Kit; Human rhinovirus receptor ELISA Kit; ICAM 1 ELISA Kit; ICAM-1 ELISA Kit; ICAM1 ELISA Kit; ICAM1_HUMAN ELISA Kit; intercellular adhesion molecule 1 (CD54) ELISA Kit; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor ELISA Kit; Intercellular adhesion molecule 1 ELISA Kit; Major group rhinovirus receptor ELISA Kit; MALA 2 ELISA Kit; MALA2 ELISA Kit; MyD 10 ELISA Kit; MyD10 ELISA Kit; P3.58 ELISA Kit; Surface antigen of activated B cells ELISA Kit; Surface antigen of activated B cells, BB2 ELISA Kit

Abbreviation

ICAM1

Uniprot No.

P05362

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Materials provided

• A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ICAM1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
• Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
• One vial Biotin-labeled ICAM1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
• One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
• One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
• One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
• One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
• One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
• One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
• One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.

Materials not provided

• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution