Human Interferon inducible T-cell Chemoattractant,I-TAC ELISA Kit
Product Details
| Target Name |
chemokine (C-X-C motif) ligand 11 |
| Alternative Names |
b R1 ELISA Kit; b-R1 ELISA Kit; Beta-R1 ELISA Kit; betaR1 ELISA Kit; C-X-C motif chemokine 11 ELISA Kit; Chemokine (C-X-C motif) ligand 11 ELISA Kit; Chemokine C-X-C motif ligand 11 ELISA Kit; CXC11 ELISA Kit; CXCL11 ELISA Kit; CXL11_HUMAN ELISA Kit; H174 ELISA Kit; I TAC ELISA Kit; I-TAC ELISA Kit; Interferon gamma inducible protein 9 ELISA Kit; Interferon gamma-inducible protein 9 ELISA Kit; Interferon inducible T cell alpha chemoattractant ELISA Kit; Interferon-inducible T-cell a chemoattractant I-TAC ELISA Kit; Interferon-inducible T-cell alpha chemoattractant ELISA Kit; IP 9 ELISA Kit; IP-9 ELISA Kit; IP9 ELISA Kit; ITAC ELISA Kit; MGC102770 ELISA Kit; SCYB11 ELISA Kit; SCYB9B ELISA Kit; Small inducible cytokine B11 ELISA Kit; Small inducible cytokine subfamily B (Cys-X-Cys) member 11 ELISA Kit; Small inducible cytokine subfamily B (Cys-X-Cys) member 9B ELISA Kit; Small inducible cytokine subfamily B; member 11 ELISA Kit; Small inducible cytokine subfamily B; member 9B ELISA Kit; Small-inducible cytokine B11 ELISA Kit |
| Abbreviation |
CXCL11 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human I-TAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
81-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
83-99 |
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| EDTA plasma (n=4) |
97 |
92-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.650 |
2.593 |
2.622 |
2.522 |
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| 1000 |
1.704 |
1.684 |
1.694 |
1.594 |
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| 500 |
0.964 |
0.982 |
0.973 |
0.873 |
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| 250 |
0.528 |
0.515 |
0.522 |
0.422 |
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| 125 |
0.330 |
0.322 |
0.326 |
0.226 |
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| 62.5 |
0.215 |
0.220 |
0.218 |
0.118 |
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| 31.25 |
0.162 |
0.169 |
0.166 |
0.066 |
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| 0 |
0.102 |
0.097 |
0.100 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
