Human Interleukin 10,IL-10 ELISA KIT
Product Details
| Target Name |
interleukin 10 |
| Alternative Names |
CSIF ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; GVHDS ELISA Kit; IL 10 ELISA Kit; IL-10 ELISA Kit; IL10 ELISA Kit; IL10_HUMAN ELISA Kit; IL10A ELISA Kit; Interleukin 10 ELISA Kit; Interleukin-10 ELISA Kit; MGC126450 ELISA Kit; MGC126451 ELISA Kit; T-cell growth inhibitory factor ELISA Kit; TGIF ELISA Kit |
| Abbreviation |
IL10 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, urine, cell culture supernates, ascitic fluid, cerebrospinal fluid (CSF), saliva |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.12 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
85-100 |
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| 1:2 |
Average % |
100 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.573 |
2.593 |
2.583 |
2.435 |
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| 400 |
2.045 |
2.000 |
2.023 |
1.875 |
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| 200 |
1.439 |
1.456 |
1.448 |
1.300 |
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| 100 |
0.880 |
0.899 |
0.890 |
0.742 |
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| 50 |
0.631 |
0.652 |
0.642 |
0.494 |
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| 25 |
0.392 |
0.421 |
0.407 |
0.259 |
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| 12.5 |
0.245 |
0.256 |
0.251 |
0.103 |
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| 0 |
0.142 |
0.153 |
0.148 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
